MEK1重组抗体[Y77]_MEK1抗体(ab32576)| Abcam中文官网 (2024)

MEK1重组抗体[Y77]_MEK1抗体(ab32576)| Abcam中文官网 (1)

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重组

  • Datasheet
  • SDS

Submit a review Submit a question References (19)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y77] to MEK1
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

MEK1重组抗体[Y77]_MEK1抗体(ab32576)| Abcam中文官网 (19) Related conjugates and formulations

Carrier Free PE

概述

  • 产品名称

    Anti-MEK1抗体[Y77]
    参阅全部 MEK1 一抗

  • 描述

    兔单克隆抗体[Y77] to MEK1

  • 宿主

    Rabbit

  • 特异性

    The antibody does not crossreact with other MAP kinase kinase family members.

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human MEK1 aa 350-450 (C terminal). The exact sequence is proprietary.

  • 阳性对照

    • WB: Wild-type HAP1 whole cell lysate; A431, Jurkat, HeLa and HepG2 whole cell lysates. IHC-P: Urinary bladder carcinoma tissue, human kidney tissue. ICC/IF: HeLa, A431 ells. IP: Jurkat cell lysate Flow Cyt (intra): HeLa cells
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production

    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

  • MEK1重组抗体[Y77]_MEK1抗体(ab32576)| Abcam中文官网 (20)Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    Y77

  • 同种型

    IgG

  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

相关产品

  • Alternative Versions

    • Anti-MEK1 antibody [Y77] - BSA and Azide free (ab167151)
    • PE Anti-MEK1 antibody [Y77] (ab213334)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human MEK1 protein (ab63209)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab32576于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)

1/30 - 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF

1/50.

WB

1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

IP

1/20 - 1/50.

IHC-P

1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

说明

Flow Cyt (Intra)
1/30 - 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/50.

WB
1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

IP
1/20 - 1/50.

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

靶标

  • 功能

    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

  • 组织特异性

    Widely expressed, with extremely low levels in brain.

  • 疾病相关

    Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.

  • 序列相似性

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.

  • 翻译后修饰

    Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
    Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

  • Information by UniProt
  • 数据库链接

  • 别名

    • Dual specificity mitogen activated protein kinase kinase 1 antibody
    • Dual specificity mitogen-activated protein kinase kinase 1 antibody
    • ERK activator kinase 1 antibody

    see all

图片

  • Western blot - Anti-MEK1 antibody [Y77] (ab32576)

    All lanes : Anti-MEK1 antibody [Y77] (ab32576) at 1/10000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : MAP2K1 knockout A549 cell lysate
    Lane 3 : Wild-type HAP1 cell lysate
    Lane 4 : MAP2K1 knockout HAP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

    Western blot: Anti-MAP2K1 antibody [Y77] (ab32576) staining at 1/10000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab32576 was shown to bind specifically to MAP2K1. A band was observed at 45 kDa in wild-type A549 cell lysates with no signal observed at this size in MAP2K1 knockout cell line. To generate this image, wild-type and MAP2K1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

  • Western blot - Anti-MEK1 antibody [Y77] (ab32576)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2:MEK1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: A431 whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32576 (unpurified) observed at 43 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab32576 was shown to specifically react with MEK1 in wild-type HAP1 cells as signal was lost in MEK1 knockout cells. Wild-type and MEK1 knockout samples were subjected to SDS-PAGE. Ab32576 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [Y77] (ab32576)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling MEK1 with purified ab32576 at 1/100 dilution (3.28 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-MEK1 antibody [Y77] (ab32576)

    All lanes : Anti-MEK1 antibody [Y77] (ab32576) at 1/10000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 antibody [Y77] (ab32576)

    Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling MEK1 with purified ab32576 at 1/50 dilution (6.6 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunoprecipitation - Anti-MEK1 antibody [Y77] (ab32576)

    ab32576 (purified) at 1/20 dilution (2ug) immunoprecipitating MEK1 in Jurkat whole cell lysate. Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10ug
    Lane 2 (+): ab32576 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32576 in Jurkat whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-MEK1 antibody [Y77] (ab32576)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MEK1 with purified ab32576 at 1/30 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-MEK1 antibody [Y77] (ab32576)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling MEK1 with purified ab32576 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [Y77] (ab32576)

    ab32576 (unpurified)at a 1/250 dilution staining MEK1 in human urinary bladder carcinoma tissue by IHC-P.

  • Western blot - Anti-MEK1 antibody [Y77] (ab32576)

    Anti-MEK1 antibody [Y77] (ab32576) at 1/10000 dilution (unpurified) + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

    Predicted band size: 43 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Anti-MEK1 antibody [Y77] (ab32576)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

    MEK1重组抗体[Y77]_MEK1抗体(ab32576)| Abcam中文官网 (32)

  • Datasheet download

    Download

文献 (19)

发表研究结果有使用 ab32576?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab32576 被引用在 19 文献中.

  • Kim M et al. Exosomes Derived from Colon Cancer Cells Promote Tumor Progression and Affect the Tumor Microenvironment. J Clin Med 12:N/A (2023). PubMed: 37373600
  • Owens AE et al. High-Throughput Cellular Thermal Shift Assay Using Acoustic Transfer of Protein Lysates. ACS Chem Biol 17:322-330 (2022). PubMed: 35119255
  • Tian J et al. MIR503HG impeded ovarian cancer progression by interacting with SPI1 and preventing TMEFF1 transcription. Aging (Albany NY) 14:5390-5405 (2022). PubMed: 35771155
  • Cao H et al. PPP1R14D promotes the proliferation, migration and invasion of lung adenocarcinoma via the PKCα/BRAF/MEK/ERK signaling pathway. Int J Oncol 61:N/A (2022). PubMed: 36263632
  • Zhang F et al. Gastric cancer cell-derived extracellular vesicles elevate E2F7 expression and activate the MAPK/ERK signaling to promote peritoneal metastasis through the delivery of SNHG12. Cell Death Discov 8:164 (2022). PubMed: 35383161

View all Publications for this product

客户评价及客户问答

MEK1重组抗体[Y77]_MEK1抗体(ab32576)| Abcam中文官网 (33)

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